1UP0
Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with cellobiose at 1.75 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.605, 93.095, 46.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.750 |
| R-factor | 0.144 |
| Rwork | 0.142 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | COMPLEX WITH SDP5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.507 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | 0.430 |
| Number of reflections | 27513 | |
| <I/σ(I)> | 14.7 | 3.8 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 5.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 10 % PEG 4K, 100 MM NA ACETATE PH 4.6, 200 MM LISO4. THE PROTEIN WAS AT 10MG/ML AND INCUBATED WITH 1 MM OF FLUORESCEINYL DERIVED 1-BETA-METHYL-CELLOTRIOSIDE-THIO-GLUCOSIDE FOR 1 HOUR PRIOR CRYSTALLISATION. 30% PEG400 WAS ADDED AS CRYOPROTECTANT |
