1UOK
CRYSTAL STRUCTURE OF B. CEREUS OLIGO-1,6-GLUCOSIDASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-06-21 |
| Detector | FUJI |
| Spacegroup name | P 62 |
| Unit cell lengths | 106.100, 106.100, 120.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24600 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.540 * |
| Data reduction software | WEIS |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.053 |
| Number of reflections | 47100 |
| <I/σ(I)> | 4.5 |
| Completeness [%] | 92.5 |
| Redundancy | 4.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | protein solution was mixed with an equal volume of reservoir solution in the drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 53 (%sat) | |
| 3 | 1 | reservoir | EDTA | 5 (mM) | |
| 4 | 1 | reservoir | potassium phosphate | 50 (mM) |
