1UOD
Crystal structure of the dihydroxyacetone kinase from E. coli in complex with dihydroxyacetone-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.498, 97.055, 86.123 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.110 - 1.900 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.718 |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.045 |
Number of reflections | 60809 |
<I/σ(I)> | 31.7 |
Completeness [%] | 94.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | pH 5.00 |