1UOC
X-ray structure of the RNase domain of the yeast Pop2 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2003-02-24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.582, 79.440, 101.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.970 - 2.300 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.26000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.309 |
| Number of reflections | 52320 | |
| <I/σ(I)> | 11.9 | 2.9 |
| Completeness [%] | 95.9 | 76.6 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | 2.5% PEG 3350,100MM HEPES(PH7.0) 80MM CALCIUM ACETATE,16.5% GLYCEROL, pH 7.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350MW | 2.5 (%) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | calcium acetate | 80 (mM) | |
| 4 | 1 | reservoir | glycerol | 16.5 (%) | |
| 5 | 1 | drop | Tris-Cl | 20 (mM) | pH7.5 |
| 6 | 1 | drop | 200 (mM) | ||
| 7 | 1 | drop | protein | 9 (mg/ml) |
