1UNV
Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 114 |
| Collection date | 2003-07-15 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 78.089, 78.089, 78.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 2.140 |
| Rwork | 0.241 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.075 |
| RMSD bond angle | 4.791 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.055 | 0.361 |
| Number of reflections | 5365 | |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 3.7 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROP, RESERVOIR: 10%W/V PEG 6K, 2 M NACL, PH 7.0 |
