1UNR
Crystal structure of the PH domain of PKB alpha in complex with a sulfate molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2002-01-15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.727, 33.963, 42.275 |
| Unit cell angles | 90.00, 119.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.250 |
| R-factor | 0.173 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| Starting model (for MR) | 1h10 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.073 | 0.394 |
| Number of reflections | 27697 | |
| <I/σ(I)> | 21.5 | 2.7 |
| Completeness [%] | 96.2 | 74.6 |
| Redundancy | 4.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS (8.5) 0.2 M AMMONIUM SULFATE, pH 8.50 |
