1UN5
ARH-II, AN ANGIOGENIN/RNASE A CHIMERA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2002-10-04 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.270, 123.973, 37.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.600 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ang |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.098 | 0.257 |
| Total number of observations | 35002 * | |
| Number of reflections | 5767 | |
| <I/σ(I)> | 19.1 | 8.3 |
| Completeness [%] | 95.1 | 98 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 | 10% PEG 6000, PH 5.4 0.2M SODIUM POTASSIUM TARTRATE, 0.02M SODIUM CITRATE BUFFER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 10 (%(w/v)) | |
| 2 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
| 3 | 1 | reservoir | citric acid/NaOH | 0.02 (M) | pH5.4 |
| 4 | 1 | drop | protein | 15-20 (mg/ml) |
