Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 1999-09-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.398, 41.533, 33.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 1.700 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b1i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.104 | 0.377 |
| Total number of observations | 107706 * | |
| Number of reflections | 13959 | |
| <I/σ(I)> | 10.8 | 9.6 |
| Completeness [%] | 97.2 | 99.7 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.2 | 15% PEG 4000, 0.02% DIOXANE, 0.2M SODIUM POTASSIUM TARTRATE, 0.02M SODIUM CITRATE BUFFER, PH 5.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | dioxane | 0.02 (%(v/v)) | |
| 3 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
| 4 | 1 | reservoir | citric acid/NaOH | 0.02 (M) | pH5.2 |
| 5 | 1 | drop | protein | 15-20 (mg/ml) |
