1UN3

CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN VARIANT T44D

Replaces:  1H0F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	CCD
Collection date	1999-09-20
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	88.398, 41.533, 33.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 1.700
R-factor	0.2
Rwork	0.199
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b1i
RMSD bond length	0.010
RMSD bond angle	1.280 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.104	0.377
Total number of observations	107706 *
Number of reflections	13959
<I/σ(I)>	10.8	9.6
Completeness [%]	97.2	99.7
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.2		15% PEG 4000, 0.02% DIOXANE, 0.2M SODIUM POTASSIUM TARTRATE, 0.02M SODIUM CITRATE BUFFER, PH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	15 (%(w/v))
2	1	reservoir	dioxane	0.02 (%(v/v))
3	1	reservoir	sodium potassium tartrate	0.2 (M)
4	1	reservoir	citric acid/NaOH	0.02 (M)	pH5.2
5	1	drop	protein	15-20 (mg/ml)