1UMZ

Xyloglucan endotransglycosylase in complex with the xyloglucan nonasaccharide XLLG.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-15
Detector	ADSC CCD
Spacegroup name	P 63
Unit cell lengths	188.162, 188.162, 45.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.000 * - 1.800
R-factor	0.203
Rwork	0.201
R-free	0.22100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1un1
RMSD bond length	0.014
RMSD bond angle	1.560 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000 *	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.063	0.662
Number of reflections	83886
<I/σ(I)>	15.4	2
Completeness [%]	96.7	71.7
Redundancy	3.61	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	293 *	10 MG ML-1 PROTEIN SOLUTION 1:1 WITH 1.0 M NAOAC AND 0.2 M IMIDAZOLE PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		1.0 (M)
3	1	reservoir	imidazole	0.2 (M)	pH6.5