1UMZ
Xyloglucan endotransglycosylase in complex with the xyloglucan nonasaccharide XLLG.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 188.162, 188.162, 45.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.000 * - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.22100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1un1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.560 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 * | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.662 |
| Number of reflections | 83886 | |
| <I/σ(I)> | 15.4 | 2 |
| Completeness [%] | 96.7 | 71.7 |
| Redundancy | 3.61 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 293 * | 10 MG ML-1 PROTEIN SOLUTION 1:1 WITH 1.0 M NAOAC AND 0.2 M IMIDAZOLE PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 1.0 (M) | ||
| 3 | 1 | reservoir | imidazole | 0.2 (M) | pH6.5 |
