1UMK
The Structure of Human Erythrocyte NADH-cytochrome b5 Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.869, 65.869, 76.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.700 - 1.750 |
| R-factor | 0.16723 |
| Rwork | 0.165 |
| R-free | 0.20680 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.025 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.700 | 1.795 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.056 | 0.143 |
| Number of reflections | 32639 | |
| <I/σ(I)> | 8.7 | 5.4 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 286 | PEG 4000, potassium phosphate, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
