1UMH
Structural basis of sugar-recognizing ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.440, 62.440, 117.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.230 - 2.000 |
| R-factor | 0.15523 |
| Rwork | 0.153 |
| R-free | 0.19229 |
| Structure solution method | MIR |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.785 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 18483 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Tris, PEG400, Nickel Chloride, Ammonium Sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
