1ULY
Crystal structure analysis of the ArsR homologue DNA-binding protein from P. horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792, 0.9795, 0.9685 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 90.640, 90.640, 132.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| Rwork | 0.204 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.083 | 0.295 |
| Number of reflections | 7284 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 10.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG400, HEPES, magnesium chloride, glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
