1ULW
Crystal structure of P450nor Ser73Gly/Ser75Gly mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.512, 79.372, 56.240 |
| Unit cell angles | 90.00, 118.18, 90.00 |
Refinement procedure
| Resolution | 26.770 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ehe |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.770 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.361 | |
| Number of reflections | 33327 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | PEG8000, 0.2M sodium acetate, sodium cacodylate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
