1ULI
Biphenyl dioxygenase (BphA1A2) derived from Rhodococcus sp. strain RHA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 137.741, 137.741, 237.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.070 - 2.200 |
R-factor | 0.16893 |
Rwork | 0.167 |
R-free | 0.20067 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.259 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.075 | 0.292 |
Number of reflections | 153515 | |
<I/σ(I)> | 8.7 | 2.5 |
Completeness [%] | 85.8 | 49 |
Redundancy | 6.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, ammonium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |