1UL9
CGL2 ligandfree
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.878, 114.615, 49.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.790 - 2.220 |
| Rwork | 0.202 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CGL2-lactose |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.790 | 2.320 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 19215 | |
| <I/σ(I)> | 21.8 | 9.8 |
| Completeness [%] | 94.5 | 66.8 |
| Redundancy | 5.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | PEG 3350, PEG 400, sodium phosphate, sodium chloride, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
