1UKR

STRUCTURE OF ENDO-1,4-BETA-XYLANASE C

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-10-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	84.300, 85.300, 113.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.179
Rwork	0.179
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRICHODERMA HARZIANUM XYLANASE PDB ENTRY 1XND.
RMSD bond length	0.011
RMSD bond angle	27.700 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BRUTE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.060
High resolution limit [Å]	2.350
Rmerge	0.082 *
Number of reflections	33131
Completeness [%]	95.3
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7	295, 277, 310 *	or sitting drop vapor diffusion or batch method *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.5 (mg/ml)
2	1	drop	sodium acetate	10 (mM)