1UKO
Crystal structure of soybean beta-amylase mutant substituted at surface region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-27 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.100, 78.140, 87.944 |
| Unit cell angles | 89.93, 89.88, 90.08 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.24080 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1byb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.249 * |
| Data reduction software | SCALE |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.240 | 2.180 |
| High resolution limit [Å] | 2.120 * | 2.120 * |
| Rmerge | 0.082 * | 0.240 * |
| Total number of observations | 314039 * | |
| Number of reflections | 99256 | 5461 * |
| Completeness [%] | 83.9 | 46.3 |
| Redundancy | 3.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 4 * | ammonium sulfate, sodium acetate, 2-mercaptoethanol, EDTA, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 45-50 (%) | |
| 3 | 1 | reservoir | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | 2-ME | 18 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.4 |
