1UKC
Crystal Structure of Aspergillus niger EstA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 63 |
| Unit cell lengths | 167.489, 167.489, 112.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.15779 |
| Rwork | 0.158 |
| R-free | 0.18412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chimera model built from 1THG and 1MAA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.287 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 104730 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 100.0 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 15% PEG 6K, 100mM AmSO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
