1UJJ

VHS domain of human GGA1 complexed with C-terminal peptide from BACE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	114.827, 114.827, 53.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.24676
Rwork	0.244
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jwg
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.059
Number of reflections	10857
<I/σ(I)>	20.7
Completeness [%]	99.4	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	PEG 5000MME, di-Ammonium hydrogen phosphate, Tris-HCl, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K