1UJ2
Crystal structure of human uridine-cytidine kinase 2 complexed with products, CMP and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.022, 109.714, 64.845 |
| Unit cell angles | 90.00, 95.32, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1udw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.046 | 0.313 |
| Number of reflections | 56389 | |
| <I/σ(I)> | 32.8 | 1.6 |
| Completeness [%] | 98.3 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG8000, calsium acetate, Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
