1UJ0

Crystal Structure of STAM2 SH3 domain in complex with a UBPY-derived peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-02
Detector	RIGAKU
Wavelength(s)	1.00
Spacegroup name	P 61
Unit cell lengths	48.454, 48.454, 58.582
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.110 - 1.700
R-factor	0.2163
Rwork	0.215
R-free	0.23000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.673
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	EPMR
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.100	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.064 *
Number of reflections	8501
Completeness [%]	97.9	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		25 *	0.08M Sodium Phosphate monobasic, 1.92M Potassium phosphate dibasic, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.6 (mg/ml)
2	1	reservoir	sodium phosphate	0.08 (M)
3	1	reservoir	potassium phosphate	1.92 (M)