1UIX
Coiled-coil structure of the RhoA-binding domain in Rho-kinase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 93 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-12-15 |
| Detector | WEISSENBERG |
| Wavelength(s) | 0.9784, 0.9778, 0.9600, 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.000, 26.100, 39.600 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 39.500 - 1.800 |
| R-factor | 0.219 |
| Rwork | 0.193 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 1.511 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.530 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.082 |
| Total number of observations | 139215 * | |
| Number of reflections | 12896 | |
| <I/σ(I)> | 18.2 | 14.8 |
| Completeness [%] | 89.0 | 68.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Thara, K., (2000) Acta Crystallogr., D56, 1042. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.7 (mg/ml) | |
| 2 | 1 | drop | HEPES | 55 (mM) | pH7.0 |
| 3 | 1 | drop | 23 (mM) | ||
| 4 | 1 | drop | PEG1000 | 11 (%) | |
| 5 | 1 | drop | ethylene glycol | 14 (%) | |
| 6 | 1 | drop | n-octanoylsucrose | 22 (mM) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 8 | 1 | reservoir | PEG1000 | 25 (%) | |
| 9 | 1 | reservoir | ethylene glycol | 30 (%) |
