1UI7
Site-directed mutagenesis of His433 involved in binding of copper ion in Arthrobacter globiformis amine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 156.510, 63.320, 185.212 |
| Unit cell angles | 90.00, 111.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.21 |
| Rwork | 0.206 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.060 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | |
| Number of reflections | 107167 | |
| Completeness [%] | 93.9 | 71.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | potassium sodium tartrate, pH 6.8, MICRODIALYSIS, temperature 289K |
