1UI6
Crystal structure of gamma-butyrolactone receptor (ArpA-like protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.792, 69.562, 148.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.22787 |
| Rwork | 0.225 |
| R-free | 0.28401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ui5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.573 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.047 | 0.205 |
| Number of reflections | 31849 | |
| <I/σ(I)> | 9 | 3.4 |
| Completeness [%] | 92.8 | 91.6 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 6000, lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
