1UHK
Crystal structure of n-aequorin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | OXFORD PX210 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 |
Unit cell lengths | 34.223, 57.038, 57.768 |
Unit cell angles | 72.35, 79.97, 67.89 |
Refinement procedure
Resolution | 33.300 - 1.600 |
R-factor | 0.1657 |
Rwork | 0.164 |
R-free | 0.19854 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.542 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.500 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 50773 | |
<I/σ(I)> | 14.5 | |
Completeness [%] | 93.4 | 79.9 |
Redundancy | 1.8 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | Ammonium sulfate, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |