1UHJ
Crystal structure of br-aequorin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.213, 56.983, 57.820 |
| Unit cell angles | 72.22, 80.32, 68.01 |
Refinement procedure
| Resolution | 28.190 - 1.800 |
| R-factor | 0.16337 |
| Rwork | 0.162 |
| R-free | 0.19819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.678 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 35673 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 95.4 | 79.9 |
| Redundancy | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammonium sulfate, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
