1UHG

Crystal Structure of S-Ovalbumin At 1.9 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-27
Detector	OXFORD PX210
Wavelength(s)	0.9
Spacegroup name	P 1
Unit cell lengths	62.647, 70.914, 83.386
Unit cell angles	87.24, 71.73, 75.95

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.196
Rwork	0.196
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ova
RMSD bond length	0.010 *
RMSD bond angle	24.300 *
Data scaling software	d*TREK
Phasing software	CCP4
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.047 *
Total number of observations	253412 *
Number of reflections	122497 *
<I/σ(I)>		2
Completeness [%]	95.5	87.2
Redundancy	2.2	2.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	30 *	Ammonium sulfate, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	90 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)	pH6.0
3	1	reservoir	ammonium sulfate	48-50 (%)
4	1	reservoir	sodium acetate	50 (mM)	pH6.0