1UHD

Crystal structure of aspartate decarboxylase, pyruvoly group bound form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9500
Spacegroup name	I 4 2 2
Unit cell lengths	81.875, 81.875, 93.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 2.000
R-factor	0.232
Rwork	0.232
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	11114
Completeness [%]	100.0	100
Redundancy	15	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K