1UHB
Crystal structure of porcine alpha trypsin bound with auto catalyticaly produced native peptide at 2.15 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.945, 53.921, 77.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.310 - 2.150 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb code 1qqu |
| RMSD bond length | 0.040 |
| RMSD bond angle | 2.597 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.140 | 0.140 |
| Number of reflections | 9847 | |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 1.5M ammonium sulphate, pH 6.70, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
