1UH3
Thermoactinomyces vulgaris R-47 alpha-amylase/acarbose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.611, 50.600, 108.555 |
| Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.600 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.730 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.087 | 0.167 |
| Total number of observations | 276350 * | |
| Number of reflections | 20035 | |
| <I/σ(I)> | 7.7 | 4.5 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.5 | 293 | Kondo, S., (2000) Protein Pept.Letters, 7, 197. * |
