1UGI
URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.800, 59.400, 79.800 |
| Unit cell angles | 73.67, 85.55, 66.46 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.222 * |
| R-free | 0.28900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ORTHORHOMBIC CRYSTAL FORM OF FREE UGI |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.064 * | 0.368 * |
| Total number of observations | 221922 * | |
| Number of reflections | 95452 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 86.7 | 86.5 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.2 | 277 | pH 8.2, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | IMIDAZOLE | ||
| 3 | 1 | 1 | MALATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 84 (%sat) | |
| 2 | 1 | reservoir | imidazole/malate | 200 (mM) |
