Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-09 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.80 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.092, 116.463, 87.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 0.990 |
| R-factor | 0.122 |
| Rwork | 0.121 |
| R-free | 0.13000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e4i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.030 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.048 | |
| Number of reflections | 305837 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.4 | 295 | PEG 4000, HEPES, Calcium chloride, pH 7.40, MICROBATCH, temperature 295.0K |
