1UFY
Crystal analysis of chorismate mutase from thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-07 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 71.252, 71.252, 152.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 0.960 |
| R-factor | 0.1099 |
| Rwork | 0.110 |
| R-free | 0.12740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PROTOMER A OF PDB ENTRY 1ODE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.960 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.050 | 0.345 |
| Number of reflections | 90841 | |
| <I/σ(I)> | 22.2 | 1.5 |
| Completeness [%] | 97.2 | 59.7 |
| Redundancy | 4.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 291 | SODIUM MALONATE, MES, pH 7.0, MICRO BATCH, temperature 291K |
