1UFL
Crystal Structure of TT1020 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.070, 97.070, 56.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.540 - 2.700 |
| Rwork | 0.230 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.060 | 0.321 |
| Total number of observations | 91310 * | |
| Number of reflections | 8743 | |
| <I/σ(I)> | 15.6 | 7.7 |
| Completeness [%] | 99.2 | 92.4 |
| Redundancy | 9.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 16 * | iso-propanol, PEG4000, Na-Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.02 (M) | |
| 3 | 1 | drop | 0.15 (M) | pH8. | |
| 4 | 1 | reservoir | iso-propanol | 7 (%) | |
| 5 | 1 | reservoir | PEG4000 | 15 (%) | |
| 6 | 1 | reservoir | sodium-Hepes | 0.05 (M) | pH7.5 |
