1UET
Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.974 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.351, 76.548, 76.771 |
| Unit cell angles | 90.00, 98.33, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.270 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.039 * | 0.168 * |
| Number of reflections | 32398 * | |
| Completeness [%] | 95.6 * | 87.3 * |
| Redundancy | 3.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 20 * | PEG4000, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 10 | 1 | reservoir | 1,6-hexanediol | 5.6 (M) | |
| 11 | 1 | reservoir | 0.4 (M) | ||
| 2 | 1 | drop | Tris-HCl | 45 (mM) | pH7.8 |
| 3 | 1 | drop | PEG4000 | 10.8 (%) | |
| 4 | 1 | drop | calcium acetate | 135 (mM) | |
| 5 | 1 | drop | 1,6-hexanediol | 6.3 (%) | |
| 6 | 1 | drop | dithiothreitol | 10 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 40 (mM) | pH7.8 |
| 8 | 1 | reservoir | PEG4000 | 9.6 (%) | |
| 9 | 1 | reservoir | calcium acetate | 120 (mM) |
