1UEI
Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, UTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-27 |
Detector | OXFORD |
Wavelength(s) | 0.9000 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 131.529, 247.748, 91.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.060 - 2.600 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1udw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.100 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.069 | 0.279 |
Number of reflections | 19587 | |
<I/σ(I)> | 8.5 | 2.2 |
Completeness [%] | 84.3 | 86.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | sodium tartrate, HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |