1UE6
Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 60.363, 117.620, 175.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ue1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.582 * |
| Number of reflections | 17174 | |
| Completeness [%] | 97.9 | 97.6 |
| Redundancy | 4.4 * | 4.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | Saikrishnan, K., (2002) Acta Cryst., D58, 327. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 3 | 1 | reservoir | 2 (M) | ||
| 4 | 1 | reservoir | sodium acetate | 1 (M) | |
| 5 | 1 | reservoir | 500 (mM) | ||
| 6 | 1 | reservoir | zinc sulfate | 50-100 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 20 (mM) | pH7.4 |
