1UE4
Crystal structure of d(GCGAAAGC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 36.907, 36.907, 64.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| Rwork | 0.209 |
| R-free | 0.23600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ue2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 | 0.330 |
| Total number of observations | 88780 * | |
| Number of reflections | 3175 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | sodium chloride, magnesium chloride, spermine 4HCl, 2-methyl-2,4-pentanediol, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium chloride | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | spermine 4HCl | ||
| 4 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
| 5 | 1 | 1 | sodium cacodylate | ||
| 6 | 1 | 2 | sodium chloride | ||
| 7 | 1 | 2 | magnesium chloride | ||
| 8 | 1 | 2 | spermine 4HCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
| 3 | 1 | drop | 12 (mM) | ||
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | MPD | 10 (%) | |
| 7 | 1 | reservoir | MPD | 25 (%) |
