1UDX

Crystal structure of the conserved protein TT1381 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-19
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	65.332, 65.332, 201.709
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.350 - 2.070
R-factor	0.226
Rwork	0.226
R-free	0.27900 *
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	23.400 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.070	2.070
Rmerge	0.044 *	0.328 *
Number of reflections	29509
Completeness [%]	99.3	100
Redundancy	5.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	20 *	MPD, Sodium acetate, sodium chloride, sodium citrate, sodium cacodylate, iso-propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.6	20 *	MPD, Sodium acetate, sodium chloride, sodium citrate, sodium cacodylate, iso-propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)	diluted with MilliQ water
2	1	reservoir	MPD	7.5 (%(v/v))
3	1	reservoir	sodium acetate	25 (mM)	pH4.6
4	1	reservoir		10 (mM)