1UDV
Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.1163 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 36.020, 134.980, 35.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.780 - 1.850 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.27400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.780 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.076 | 0.229 |
| Number of reflections | 15639 | |
| <I/σ(I)> | 15.9 | 5.8 |
| Completeness [%] | 99.5 | 92.6 * |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 298 | PEG MME 550, ZnSO4, MES, pH 6.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG550 MME | 25 (%) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
