1UDI
NUCLEOTIDE MIMICRY IN THE CRYSTAL STRUCTURE OF THE URACIL-DNA GLYCOSYLASE-URACIL GLYCOSYLASE INHIBITOR PROTEIN COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 65 |
| Unit cell lengths | 144.120, 144.120, 40.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.27600 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.468 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.700 * | |
| Rmerge | 0.070 | |
| Number of reflections | 12683 | |
| Completeness [%] | 92.7 | 85 * |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 11 (%(w/v)) | |
| 2 | 1 | 1 | sodium phosphate | 100 (mM) | |
| 3 | 1 | 1 | ammonium sulfate | 35 (mM) | |
| 4 | 1 | 1 | protein | 12.5 (mg/ml) |
