1UDA

STRUCTURE OF UDP-GALACTOSE-4-EPIMERASE COMPLEXED WITH UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	83.500, 83.500, 108.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.188 *
Rwork	0.188
RMSD bond length	0.015
RMSD bond angle	14.900 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.880
High resolution limit [Å]	1.800 *	1.800
Rmerge	0.043	0.216
Total number of observations	120477 *
Number of reflections	39033	4283 *
Completeness [%]	92.0	83
Redundancy	3.09

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20-25 (%)
2	1	reservoir		250-500 (mM)
3	1	reservoir	CHES	50 (mM)