1UD9

Crystal Structure of Proliferating Cell Nuclear Antigen (PCNA) Homolog From Sulfolobus tokodaii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-17
Detector	MARRESEARCH
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	79.400, 75.440, 88.140
Unit cell angles	90.00, 98.83, 90.00

Refinement procedure

Resolution	10.000 - 1.680
Rwork	0.202
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	2.090
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.050	0.398
Number of reflections	114482
<I/σ(I)>	10.2
Completeness [%]	97.7	82.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	3.75%(w/v) PEG8000, 0.1M MES, 0.2M Zn(OAc)2, 0.1M Glycine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K