1UCX
Crystal structure of proglycinin C12G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-09-18 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 115.201, 115.201, 147.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.218 |
| Rwork | 0.180 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 3.000 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.400 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.141 * | 0.523 * |
| Number of reflections | 30085 * | |
| <I/σ(I)> | 8.75 | 2.46 |
| Completeness [%] | 97.4 | 95.6 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 5 * | Gidamis, A.B., (1994) Biosci., Biotechnol., Biochem., 58, 703. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 35 (mM) | pH7.6 |
| 3 | 1 | drop | 0.15 (M) | ||
| 4 | 1 | drop | PMSF | 1.5 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | 2ME | 10 (mM) | |
| 7 | 1 | drop | 0.02 (%(w/v)) | ||
| 8 | 1 | reservoir | PEG6000 | 3-12 (%(w/v)) |
