1UCQ

Crystal structure of the L intermediate of bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 6 2 2
Unit cell lengths	102.300, 102.300, 112.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.300 * - 2.400
Rwork	0.300
R-free	0.33200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iw6
RMSD bond length	0.008
RMSD bond angle	1.280 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.066 *
Number of reflections	13880
<I/σ(I)>	5.3	1.5
Completeness [%]	99.8	49.2 *
Redundancy	5.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	5 *	ammonium sulfate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 278.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	octylthioglucoside	2.5 (mg/ml)
2	1	drop	ammonium sulfate	1.0 (M)
3	1	drop		0.16 (M)
4	1	drop	trehalose	12 (%)
5	1	drop		0.04 (%)
6	1	drop	sodium citrate	0.08 (M)	pH5.2
7	1	reservoir	ammonium sulfate	1.9-2.0 (M)