1UCQ
Crystal structure of the L intermediate of bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 102.300, 102.300, 112.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.300 * - 2.400 |
| Rwork | 0.300 |
| R-free | 0.33200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iw6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 * | |
| Number of reflections | 13880 | |
| <I/σ(I)> | 5.3 | 1.5 |
| Completeness [%] | 99.8 | 49.2 * |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 5 * | ammonium sulfate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 278.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | octylthioglucoside | 2.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | drop | 0.16 (M) | ||
| 4 | 1 | drop | trehalose | 12 (%) | |
| 5 | 1 | drop | 0.04 (%) | ||
| 6 | 1 | drop | sodium citrate | 0.08 (M) | pH5.2 |
| 7 | 1 | reservoir | ammonium sulfate | 1.9-2.0 (M) |
