1UCG

Crystal structure of Ribonuclease MC1 N71T mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	58.790, 135.900, 52.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.650
Rwork	0.181
R-free	0.19500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bk7
RMSD bond length	0.004
RMSD bond angle	1.260 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.810 *	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.053	0.240 *
Total number of observations	434769 *
Number of reflections	50905 *
<I/σ(I)>	9.1	3.8
Completeness [%]	99.2	97.6 *
Redundancy	8.5 *	5.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	PEG 1540, Manganese Cloride tetrahydrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12-16 (mg/ml)
2	1	reservoir		10 (mM)
3	1	reservoir	PEG1540	30 (%(w/v))