1UCF
The Crystal Structure of DJ-1, a Protein Related to Male Fertility and Parkinson's Disease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.039, 75.039, 74.877 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.520 - 1.950 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.19400 * |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.338 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.399 |
| Total number of observations | 145149 * | |
| Number of reflections | 34488 | |
| Completeness [%] | 98.2 | 93.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | sodium citrate, HEPES, DTT, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
