1UCA
Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 2'-UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1999-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.810, 67.940, 75.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.480 |
| Rwork | 0.194 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bk7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.658 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.075 | 0.500 |
| Total number of observations | 335854 * | |
| Number of reflections | 31397 | |
| <I/σ(I)> | 6.8 | 1.5 |
| Completeness [%] | 92.9 | 91.7 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG 8000, sodium acetate, sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.7 |
| 4 | 1 | reservoir | PEG8000 | 21 (%) | |
| 5 | 1 | reservoir | 2'-UMP | 10 (mM) |
