1UC2
Hypothetical Extein Protein of PH1602 from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.251, 137.586, 148.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.150 |
| Rwork | 0.168 |
| R-free | 0.20400 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.598 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.390 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.085 | 0.345 |
| Number of reflections | 90861 | |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Sodium Chloride, ammonium sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
