1UC0

Crystal structure of wild-type hen-egg white lysozyme singly labeled with 2',3'-epoxypropyl beta-glycoside of N-acetyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	283
Detector technology	AREA DETECTOR
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.040, 78.040, 38.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.850
Rwork	0.190
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Wild-type Hen-egg white lysozyme
RMSD bond length	0.009
RMSD bond angle	1.620
Data reduction software	Harata's (program)
Data scaling software	MERGEF ((K.HARATA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.600	1.890
High resolution limit [Å]	1.780	1.850
Rmerge	0.048	0.258
Total number of observations	74099 *
Number of reflections	10897 *
Completeness [%]	90.8
Redundancy	6.80 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5	298	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	50 (mM)	pH4.5